Status: Success


Big Trouble In Little China

Mission Objectives

do science! like the real kind
create new cultures
salvage Dozer's lab experiment
avoid becoming a lab rat

Mission Summary

This mission was in serious risk of being PurposelessFun, until Maggot
Dozer gave us a purpose - in a lab!

We rode through several
star systems and constellations, diffused a possible situation with an
unruly civilian on a trans-system stargate, encountered an odd caped
fellow, and saw frogs. Lab work done, we headed down to the water,
derbied a bit, and went hat fishing. Rubbish nearly killed a rat, but
was probably more traumatized than the rat from the incident.

After departing the derby/hat fishing/rat-squishing location,
we flew through a part of the Cambridge/Somerville Systems which
contained a thick cup belt, and there was much rejoicing (and
crunching noises). Once back at the landing pad, Ehawk was knighted as
a Pilot, and there was much more rejoicing.

Also, Nosepicker
got donuts.

BabyMaggot Automatic was tended to by Masokist.

Additional Report From Dozer

Just wanted to inform you, SIR, that The mission REALLY WAS a success!
DNA of the Right Sort was isolated from cultures that were inoculated
from those agar plates we rescued Saturnight. Had I left the plates
until the next day, the selective agent, in this case ampicillin, would
have been depleted by the resistant bacteria (bearing My Favorite DNA)
and the non-resistant bacteria (not bearing my Favorite DNA) would have
taken over like a horde of chopper riding, Schlitz swilling, skinny
dippin' anarchistic bacteria. Hmm. Perhaps that wouldn't have been so

Really Geeky Explanation Below: Do not read if you are drowsy or taking

The medium in the agar plates you saw included the antibiotic
ampicillin. My Favorite DNA, encoding the potassium channel whose
function I'm studying, was previously inserted into a circular bit of
bacterial DNA that enables a bacterium to grow on ampicillin plates.
Some innocent virgin bacteria were then exposed to this DNA. Some took
it up, some didn't. This mixed population was spread onto those plates
you saw. But I could select the men from the boys, so to speak, on this
principal: No Amp gene, no growth.

Small samples of the E coli colonies (strain DH5 alpha, NOT O157:H7)
growing on the plates you saw were transferred to 5 mL of liquid
culture medium and grown over night. The cultures were centrifuged,
which concentrates the bacteria into tight pellets at the bottom of the
tubes. DNA was then extracted from the bacterial pellets and purified.
I ran an analytical digest, using two enzymes that I know will cut the
circular DNA at two defined sites. The fragments of DNA in the
digestion reaction were separated according to molecular weight by gel
electrophoresis. DNA bears a net negative charge and will migrate
toward the positive terminal of a gel soaking in a bath of buffer
across which an electrical potential has been applied. The resultant
pattern was consistent with that of DNA encoding My Favorite Protein.

End Geekiness Here
Pilot Ship Points Promotion
AceHole Bonnie & Clyde 503.301   Captain
dillweed 100Hz 181.344  
eHawk Devastation 444.13   Petty Officer First Class
Exon Anger 303.831   Lieutenant Commander
GRIMLOCKE Doctor Love 356.767  
Hapto Ez Raider 177.644  
Iscariot Sloth 1236.04   Admiral
Mars High Hard One 239.445  
Masokist Toxic Shock 864.35  
Meatball Mulligan Ooh La La 235.634   Ensign
moose Loki Ducker 1209.6   Rear Admiral, Upper Half
Mosquito Famine 281.604   Commodore
Nosepicker Pale Horse 456.057  
pywaket Circus Peanut 411.846   Lieutenant
Rotwang Vertigo 526.578   Captain
Rubbish Spooky Pirate 600.529   Commander
Secret Chief Mjollnir 597.856   Commander
Skunk Delirium 1876.97  
Threespeed Schadenfreude 479.932  
Pilots must be logged in to see the briefing.
Taskforce Pilot
Mission Leader Skunk
Deck Officer Skunk
Navigator Skunk
Tailgunner Rubbish
Still Cam Skunk
Life Support 1 Skunk
Mission Reporter Dozer
Filmer Masokist

Mission Parameters

Mission LeaderSkunk
Mission Size 19 pilots
OriginFort Summer
DestinationThe Lab
Light Years21.520
G-Well Activity2.014
Technical Rating2.835